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- Title
Production of isopropanol by metabolically engineered Escherichia coli.
- Authors
Jojima, Toru; Inui, Masayuki; Yukawa, Hideaki
- Abstract
A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary-secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions.
- Publication
Applied microbiology and biotechnology, 2008, Vol 77, Issue 6, p1219
- ISSN
0175-7598
- Publication type
Journal Article
- DOI
10.1007/s00253-007-1246-8