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- Title
Structured illumination microscopy of a living cell.
- Authors
Hirvonen, Liisa M; Wicker, Kai; Mandula, Ondrej; Heintzmann, Rainer
- Abstract
Due to diffraction, the resolution of imaging emitted light in a fluorescence microscope is limited to about 200 nm in the lateral direction. Resolution improvement by a factor of two can be achieved using structured illumination, where a fine grating is projected onto the sample, and the final image is reconstructed from a set of images taken at different grating positions. Here we demonstrate that with the help of a spatial light modulator, this technique can be used for imaging slowly moving structures in living cells.
- Publication
European biophysics journal : EBJ, 2009, Vol 38, Issue 6, p807
- ISSN
1432-1017
- Publication type
Journal Article
- DOI
10.1007/s00249-009-0501-6