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- Title
Cholesterol inhibits the insertion of the Alzheimer's peptide Abeta(25-35) in lipid bilayers.
- Authors
Dante, Silvia; Hauss, Thomas; Dencher, Norbert A
- Abstract
The physiological relationship between brain cholesterol content and the action of amyloid beta (Abeta) peptide in Alzheimer's disease (AD) is a highly controversially discussed topic. Evidences for modulations of the Abeta/membrane interaction induced by plasma membrane cholesterol have already been observed. We have recently reported that Abeta(25-35) is capable of inserting in lipid membranes and perturbing their structure. Applying neutron diffraction and selective deuteration, we now demonstrate that cholesterol alters, at the molecular level, the capability of Abeta(25-35) to penetrate into the lipid bilayers; in particular, a molar weight content of 20% of cholesterol hinders the intercalation of monomeric Abeta(25-35) completely. At very low cholesterol content (about 1% molar weight) the location of the C-terminal part of Abeta(25-35) has been unequivocally established in the hydrocarbon region of the membrane, in agreement with our previous results on pure phospholipids membrane. These results link a structural property to a physiological and functional behavior and point to a therapeutical approach to prevent the AD by modulation of membrane properties.
- Publication
European biophysics journal : EBJ, 2006, Vol 35, Issue 6, p523
- ISSN
0175-7571
- Publication type
Journal Article
- DOI
10.1007/s00249-006-0062-x