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- Title
Hydrogen peroxide induces apoptosis of osteocytes: involvement of calcium ion and caspase activity.
- Authors
Kikuyama, A; Fukuda, K; Mori, S; Okada, M; Yamaguchi, H; Hamanishi, C
- Abstract
We hypothesized that reactive oxygen species play an important role in avascular/ischemic osteonecrosis. When isolated chick osteocytes were cultured with hydrogen peroxide, annexin V binding, which is the earliest marker of apoptosis, increased in a dose-dependent fashion. Hydrogen peroxide also induced the activation of caspase-3 and increase in cytosolic Ca2+. Treatment with BAPTA/AM (cheletor of cytosolic Ca2+) and Ac-DEVD-cho (caspase inhibitor) attenuated hydrogen peroxide-induced apoptosis. These data demonstrated the signal transduction pathways that participate in this hydrogen peroxide-induced cell damage.
- Publication
Calcified tissue international, 2002, Vol 71, Issue 3, p243
- ISSN
0171-967X
- Publication type
Journal Article
- DOI
10.1007/s00223-001-1110-2