Studies into the phenolic patterns of different tissues of pineapple ( Ananas comosus [L.] Merr.) infructescence by HPLC-DAD-ESI-MS and GC-MS analysis.

Steingass, Christof; Glock, Mona; Schweiggert, Ralf; Carle, Reinhold

In a comprehensive study, more than 60 phenolic compounds were detected in methanolic extracts from different tissues of pineapple infructescence by high-performance liquid chromatography with diode array detection and electrospray ionisation multiple-stage mass spectrometry (HPLC-DAD-ESI-MS) as well as by gas chromatography-mass spectrometry (GC-MS). The analytical workflow combining both methods revealed numerous compounds assigned for the first time as pineapple constituents by their mass fragmentations. Pineapple crown tissue was characterised by depsides of p-coumaric and ferulic acid. In contrast, major phenolic compounds in pineapple pulp extracts were assigned to diverse S- p-coumaryl, S-coniferyl and S-sinapyl derivatives of glutathione, N- l-γ-glutamyl- l-cysteine and l-cysteine, which were also identified in the peel. The latter was additionally characterised by elevated concentrations of p-coumaric, ferulic and caffeic acid depsides and glycerides, respectively. Two peel-specific cyanidin hexosides were found. Elevated concentrations of isomeric N, N′-diferuloylspermidines may be a useful tool for the detection of fraudulent peel usage for pineapple juice production. Mass fragmentation pathways of characteristic pineapple constituents are proposed, and their putative biological functions are discussed.

FOOD chemistry; PHENOL content of fruit; GLUTATHIONE; PINEAPPLE; FRUIT extracts; HIGH performance liquid chromatography

Analytical & Bioanalytical Chemistry, 2015, Vol 407, Issue 21, p6463

1618-2642

Academic Journal

10.1007/s00216-015-8811-2