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- Title
Aptamer-based electrochemical biosensor for Botulinum neurotoxin.
- Authors
Fang Wei; Chih-Ming Ho
- Abstract
We have developed an aptamer-based electrochemical sensor for detection of Botulinum neurotoxin, where steric hindrance is applied to achieve specific signal amplification via conformational change of the aptamer. The incubation time and potassium concentration of the reaction buffer were found to be key parameters affecting the sensitivity of detection of the recognition of Botulinum neurotoxin by the aptamer. Under optimized experimental conditions, a high signal-to-noise ratio was obtained within 24 h with a limit of detection (LOD) of 40 pg/ml by two standard deviation cutoffs above the noise level.
- Publication
Analytical & Bioanalytical Chemistry, 2009, Vol 393, Issue 8, p1943
- ISSN
1618-2642
- Publication type
Academic Journal
- DOI
10.1007/s00216-009-2687-y