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- Title
Time-domain fluorescence lifetime imaging for intracellular pH sensing in living tissues.
- Authors
Hille, Carsten; Berg, Maik; Bressel, Lena; Munzke, Dorit; Primus, Philipp; Löhmannsröben, Hans-Gerd; Dosche, Carsten
- Abstract
pH sensing in living cells represents one of the most prominent topics in biochemistry and physiology. In this study we performed one-photon and two-photon time-domain fluorescence lifetime imaging with a laser-scanning microscope using the time-correlated single-photon counting technique for imaging intracellular pH levels. The suitability of different commercial fluorescence dyes for lifetime-based pH sensing is discussed on the basis of in vitro as well of in situ measurements. Although the tested dyes are suitable for intensity-based ratiometric measurements, for lifetime-based techniques in the time-domain so far only BCECF seems to meet the requirements of reliable intracellular pH recordings in living cells.
- Publication
Analytical and bioanalytical chemistry, 2008, Vol 391, Issue 5, p1871
- ISSN
1618-2650
- Publication type
Journal Article
- DOI
10.1007/s00216-008-2147-0