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- Title
Fine mapping a QTL for carbon isotope composition in tomato.
- Authors
Xiangyang Xu; Martin, Bjorn; Comstock, Jonathan P.; Vision, Todd J.; Tauer, Charles G.; Baige Zhao; Pausch, Roman C.; Knapp, Steven
- Abstract
Carbon isotope composition (δ13C) and leaf water-use efficiency vary in concert in C3 plants, making δ13C useful as a proxy for plant water-use efficiency. A QTL for δ13C was detected in the Solanum pennellii chromosome fragment of IL5-4, an introgression line with S. lycopersicum cv. M82 background. M82 and IL 5-4 were crossed, and RFLP markers in the target region converted to PCR-based markers. Forty-one recombinants with an introgression fragment ranging in length from 1.1 to 11.4 cM were identified by marker assisted selection (MAS) among approximately 2000 F2 plants. A total of 29 markers were mapped within the introgression fragment unique to IL5-4. These markers divided the about 9 cM target region into nine intervals. A dominant QTL for δ13C, designated QWUE5.1 that explained 25.6% of the total phenotypic variance was mapped to an interval about 2.2 cM long. Twenty-one plants with a S. pennellii chromosome fragment shortened to a length of 2.0–9.1 cM by a second recombination event were generated by MAS of 1,125 F4 plants. Two near isogenic lines with high δ13C (small negative value) and carrying QWUE5.1 on the shortest introgression fragments (about 7.0 cM) were identified. The markers and genetic stocks developed are valuable for cloning the gene underlying QWUE5.1, MAS of QWUE5.1, and fine-mapping genes/QTL located in this region.
- Publication
Theoretical & Applied Genetics, 2008, Vol 117, Issue 2, p221
- ISSN
0040-5752
- Publication type
Academic Journal
- DOI
10.1007/s00122-008-0767-6