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- Title
Comparative analysis of polymorphism and chromosomal location of tomato microsatellite markers isolated from different sources.
- Authors
Areshchenkova, T.; Ganal, M. W.
- Abstract
Previously isolated tomato ( Lycopersicon esculentum) microsatellite markers were mainly clustered in the centromeric heterochromatin and not located in euchromatic regions. To achieve a more-uniform distribution of microsatellite markers for genome mapping purposes, a set of tomato microsatellite markers containing dinucleotide simple sequence repeats were developed by screening genomic libraries enriched for single-copy sequences, and screening the tomato EST database. The tomato microsatellites isolated in these ways were characterized by combinations of different types of repeated motifs and they were polymorphic in a set of L. esculentum varieties detecting up to four alleles. A total of 20 markers were placed on the genetic map of tomato. Interestingly, all markers isolated from genomic libraries enriched for single-copy sequences by PstI-pre-digestion mapped into the centromeric regions. The majority of markers derived from EST sequences contained predominantly AT microsatellites and were located in euchromatic regions.
- Publication
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2002, Vol 104, Issue 2-3, p229
- ISSN
1432-2242
- Publication type
Journal Article
- DOI
10.1007/s00122-001-0775-2