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- Title
Benzyl isothiocyanate exhibits anti-inflammatory effects in murine macrophages and in mouse skin.
- Authors
Yeo Myeong Lee; Mi Ra Seon; Han Jin Cho; Jong-Sang Kim; Jung Yoon Park
- Abstract
Benzyl isothiocyanate (BITC) is detected in abundance in Brassica vegetables, and some previous studies have demonstrated that BITC may potentially function as a chemopreventive agent in humans. This study examined whether BITC inhibits lipopolysaccharide (LPS)-induced inflammatory responses in Raw 264.7 macrophages and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema formation. The treatment of macrophages with various concentrations of BITC resulted in a dose-dependent reduction in the LPS-induced secretion of interleukin (IL)-1β, TNF-α, and IL-6 and their corresponding mRNA levels, as well as in the production of nitric oxide and PGE2. Consistent with these findings, BITC inhibited the LPS-induced expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 proteins and their corresponding mRNAs. BITC inhibited LPS-induced phosphorylation and the degradation of the inhibitor of κBα, translocation of p65 into the nucleus, and the DNA binding activity and transcriptional activity of NFκB. Moreover, the LPS-stimulated phosphorylation of extracellular signal regulated kinase (ERK)1/2 and Akt was suppressed by BITC. BITC also inhibited ear edema formation and the protein expression of iNOS and COX-2 in mouse skin treated with TPA. We demonstrate that BITC is a potent anti-inflammatory agent, and the anti-inflammatory properties of BITC may result from the downregulation of NFκB signaling.
- Publication
Journal of Molecular Medicine, 2009, Vol 87, Issue 12, p1251
- ISSN
0946-2716
- Publication type
Academic Journal
- DOI
10.1007/s00109-009-0532-6