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- Title
Technologies of directed protein evolution in vivo.
- Authors
Blagodatski, Artem; Katanaev, Vladimir L
- Abstract
Directed evolution of proteins for improved or modified functionality is an important branch of modern biotechnology. It has traditionally been performed using various in vitro methods, but more recently, methods of in vivo artificial evolution come into play. In this review, we discuss and compare prokaryotic and eukaryotic-based systems of directed protein evolution in vivo, highlighting their benefits and current limitations and focusing on the biotechnological potential of vertebrate immune cells for the generation of protein diversity by means of the immunoglobulin diversification machinery.
- Publication
Cellular and molecular life sciences : CMLS, 2011, Vol 68, Issue 7, p1207
- ISSN
1420-9071
- Publication type
Journal Article
- DOI
10.1007/s00018-010-0610-5