We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bone marrow-derived mesenchymal cells and MMP13 contribute to experimental choroidal neovascularization.
- Authors
Lecomte, Julie; Louis, Krystel; Detry, Benoit; Blacher, Silvia; Lambert, Vincent; Bekaert, Sandrine; Munaut, Carine; Paupert, Jenny; Blaise, Pierre; Foidart, Jean-Michel; Rakic, Jean-Marie; Krane, Stephen M; Noel, Agnès
- Abstract
In this study, we evaluate the potential involvement of collagenase-3 (MMP13), a matrix metalloproteinase (MMP) family member, in the exudative form of age-related macular degeneration characterized by a neovascularisation into the choroid. RT-PCR analysis revealed that human neovascular membranes issued from patients with AMD expressed high levels of Mmp13. The contribution of MMP13 in choroidal neovascularization (CNV) formation was explored by using a murine model of laser-induced CNV and applying it to wild-type mice (WT) and Mmp13-deficient mice (Mmp13 ( -/- ) mice). Angiogenic and inflammatory reactions were explored by immunohistochemistry. The implication of bone marrow (BM)-derived cells was determined by BM engraftment into irradiated mice and by injecting mesenchymal stem cells (MSC) isolated from WT BM. The deficiency of Mmp13 impaired CNV formation which was fully restored by WT BM engraftment and partially rescued by several injections of WT MSC. The present study sheds light on a novel function of MMP13 during BM-dependent choroidal vascularization and provides evidence for a role for MSC in the pathogenesis of CNV.
- Publication
Cellular and molecular life sciences : CMLS, 2011, Vol 68, Issue 4, p677
- ISSN
1420-9071
- Publication type
Journal Article
- DOI
10.1007/s00018-010-0476-6