Your institution may have access to this item. Find your institution then sign in to continue.

Title: A Protocol for High-Throughput Extraction of DNA From Rice Leaves.
Authors: Xin Xu; Kawasaki, Shinji; Fujimura, Tatsuhito; Chuntai Wang
Abstract: Current DNA isolation methods have limitations between speed and purity in high-throughput molecular genetic analysis such as gene mapping and marker-assisted selection programs. We have optimized a simple and rapid method for isolating high-quality genomic DNA from rice that significantly minimizes time and the use of laboratory materials. One person can process as many as 384 samples in 2 h. The isolated DNA is suitable for polymerase chain reaction-based techniques and is stable for no less than 6 mo of storage at 4°C.
Subjects: DNA; GENE mapping; CLONING; GENETIC techniques; GENOMICS
Publication: Plant Molecular Biology Reporter, 2005, Vol 23, Issue 3, p291
ISSN: 0735-9640
Publication type: Academic Journal
DOI: 10.1007/BF02772759

We found a match

A Protocol for High-Throughput Extraction of DNA From Rice Leaves.