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- Title
Lack of ABCG2 Expression and Side Population Properties in Human Pluripotent Stem Cells.
- Authors
Hui Zeng; Jung Woo Park; Min Guo; Ge Lin; Crandall, Leann; Compton, Tiwanna; Xiaofang Wang; Xue-Jun Li; Fang-Ping Chen; Ren-He Xu
- Abstract
The multidrug transporter ABCG2 in cell membranes enables various stem cells and cancer cells to efflux chemicals, including the fluorescent dye Hoechst 33342. The Hoechst" cells can be sorted out as a side population with stem cell properties. Abcg2 expression in mouse embryonic stem cells (ESCs) reduces accumulation of DNA-damaging metabolites in the cells, which helps prevent cell differentiation. Surprisingly, we found that human ESCs do not express ABCG2 and cannot efflux Hoechst. In contrast, trophoblasts and neural epithelial cells derived from human ESCs are ABCG2+ and Hoechst". Human ESCs ectopically expressing ABCG2 become Hoechst", more tolerant of toxicity of mitoxantronΘ, a substrate of ABCG2, and more capable of self-renewal in basic flbroblast growth factor (bFGF)-free condition than control cells. However, Hoechst'"w cells sorted as a small subpopulation from human ESCs express lower levels of pluripotency markers than the Hoechsth'8h cells. Similar results were observed with human induced plu ri potent stem cells. Conversely, mouse ESCs are Abcg2+ and mouse trophoblasts, Abcg2~. Thus, absence of ABCG2 is a novel feature of human pluripotent stem cells, which distinguishes them from many other stem cells including mouse ESCs, and may be a reason why they are sensitive to suboptimal culture conditions. Stem Cells 2009í27:2435-2445
- Publication
Stem Cells, 2009, Vol 27, Issue 10, p2435
- ISSN
1066-5099
- Publication type
Academic Journal
- DOI
10.1002/stem.192