We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Nanostructured superhydrophobic substrates trigger the development of 3D neuronal networks.
- Authors
Limongi, Tania; Cesca, Fabrizia; Gentile, Francesco; Marotta, Roberto; Ruffilli, Roberta; Barberis, Andrea; Dal Maschio, Marco; Petrini, Enrica Maria; Santoriello, Stefania; Benfenati, Fabio; Di Fabrizio, Enzo
- Abstract
The generation of 3D networks of primary neurons is a big challenge in neuroscience. Here, a novel method is presented for a 3D neuronal culture on superhydrophobic (SH) substrates. How nano-patterned SH devices stimulate neurons to build 3D networks is investigated. Scanning electron microscopy and confocal imaging show that soon after plating neurites adhere to the nanopatterned pillar sidewalls and they are subsequently pulled between pillars in a suspended position. These neurons display an enhanced survival rate compared to standard cultures and develop mature networks with physiological excitability. These findings underline the importance of using nanostructured SH surfaces for directing 3D neuronal growth, as well as for the design of biomaterials for neuronal regeneration.
- Publication
Small (Weinheim an der Bergstrasse, Germany), 2013, Vol 9, Issue 3, p402
- ISSN
1613-6829
- Publication type
Journal Article
- DOI
10.1002/smll.201201377