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- Title
The catalytic domain of the germination-specific lytic transglycosylase SleB from Bacillus anthracis displays a unique active site topology.
- Authors
Jing, Xing; Robinson, Howard R; Heffron, Jared D; Popham, David L; Schubot, Florian D
- Abstract
Bacillus anthracis produces metabolically inactive spores. Germination of these spores requires germination-specific lytic enzymes (GSLEs) that degrade the unique cortex peptidoglycan to permit resumption of metabolic activity and outgrowth. We report the first crystal structure of the catalytic domain of a GSLE, SleB. The structure revealed a transglycosylase fold with unique active site topology and permitted identification of the catalytic glutamate residue. Moreover, the structure provided insights into the molecular basis for the specificity of the enzyme for muramic-δ-lactam-containing cortex peptidoglycan. The protein also contains a metal-binding site that is positioned directly at the entrance of the substrate-binding cleft.
- Publication
Proteins, 2012, Vol 80, Issue 10, p2469
- ISSN
1097-0134
- Publication type
Journal Article
- DOI
10.1002/prot.24140