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- Title
Genome-wide DNA methylation profiles in hepatocellular carcinoma.
- Authors
Shen, Jing; Wang, Shuang; Zhang, Yu-Jing; Kappil, Maya; Wu, Hui-Chen; Kibriya, Muhammad G; Wang, Qiao; Jasmine, Farzana; Ahsan, Habib; Lee, Po-Huang; Yu, Ming-Whei; Chen, Chien-Jen; Santella, Regina M
- Abstract
Alterations in DNA methylation frequently occur in hepatocellular cancer (HCC). We have previously demonstrated that hypermethylation in candidate genes can be detected in plasma DNA before HCC diagnosis. To identify, with a genome-wide approach, additional genes hypermethylated in HCC that could be used for more accurate analysis of plasma DNA for early diagnosis, we analyzed tumor and adjacent nontumor tissues from 62 Taiwanese HCC cases using Illumina methylation arrays (Illumina, Inc., San Diego, CA) that screen 26,486 autosomal CpG sites. After Bonferroni adjustment, a total of 2,324 CpG sites significantly differed in methylation level, with 684 CpG sites significantly hypermethylated and 1,640 hypomethylated in tumor, compared to nontumor tissues. Array data were validated with pyrosequencing in a subset of five of these genes; correlation coefficients ranged from 0.92 to 0.97. Analysis of plasma DNA from 38 cases demonstrated that 37%-63% of cases had detectable hypermethylated DNA (≥ 5% methylation) for these five genes individually. At least one of these genes was hypermethylated in 87% of the cases, suggesting that measurement of DNA methylation in plasma samples is feasible.
- Publication
Hepatology (Baltimore, Md.), 2012, Vol 55, Issue 6, p1799
- ISSN
1527-3350
- Publication type
Journal Article
- DOI
10.1002/hep.25569