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- Title
C. elegans nuclear receptor NHR-6 functionally interacts with the jun-1 transcription factor during spermatheca development.
- Authors
Gissendanner, Chris R; Cardin, Derrick; Dubose, Chris J; El Sayed, Moustafa; Harmson, Jeremy S; Praslicka, Brandon; Rowan, Brian G
- Abstract
The NR4A nuclear receptor NHR-6 is an essential regulator of spermatheca organogenesis in C. elegans. In this study, we perform a focused, RNAi-based screen to identify modifiers of partial nhr-6 loss of function. Ninety-eight genes that encode signaling proteins expressed in the spermatheca were screened for enhancement of the nhr-6 RNAi phenotype. We identify the C. elegans gene jun-1, which encodes the homolog of the Jun transcription factor, as a strong enhancer of nhr-6 partial loss of function. We show that nhr-6 and jun-1 function together to regulate development of the spermatheca and are necessary for generating an organ with the normal number of cells. jun-1 is expressed in all cells of the developing spermatheca. We also provide evidence that NHR-6 and JUN-1 can physically interact in a yeast two-hybrid assay. Our results provide in vivo evidence that NR4A nuclear receptor and Jun transcription factor interactions are essential in regulating developmental processes in metazoans.
- Publication
Genesis (New York, N.Y. : 2000), 2014, Vol 52, Issue 1, p29
- ISSN
1526-968X
- Publication type
Research
- DOI
10.1002/dvg.22723