Title

Cytochrome P450 119 Compounds I Formed by Chemical Oxidation and Photooxidation Are the Same Species.

Authors

Su, Zhi; Horner, John H.; Newcomb, Martin

Abstract

Compound I from cytochrome P450 119 prepared by the photooxidation method involving peroxynitrite oxidation of the resting enzyme to Compound II followed by photooxidation to Compound I was compared to Compound I generated by m‐chloroperoxybenzoic acid (MCPBA) oxidation of the resting enzyme. The two methods gave the same UV/Visible spectra, the same products from oxidations of lauric acid and palmitic acid and their (ω‐2,ω‐2,ω‐3,ω‐3)‐tetradeuterated analogues, and the same kinetics for oxidations of lauric acid and caprylic acid. The experimental identities between the transients produced by the two methods leave no doubt that the same Compound I species is formed by the two methods.

Subjects

CYTOCHROME P-450; OCTANOIC acid; LAURIC acid; OXIDATION; PALMITIC acid; PHOTOOXIDATION; CYTOCHROME oxidase

Publication

Chemistry - A European Journal, 2019, Vol 25, Issue 61, p14015

ISSN

0947-6539

Publication type

Academic Journal

DOI

10.1002/chem.201202254