Title

Kinetic Resolution of Racemic Primary Amines Using Geobacillus stearothermophilus Amine Dehydrogenase Variant.

Authors

Tseliou, Vasilis; Knaus, Tanja; Vilím, Jan; Masman, Marcelo F.; Mutti, Francesco G.

Abstract

A NADH‐dependent engineered amine dehydrogenase from Geobacillus stearothermophilus (LE‐AmDH‐v1) was applied together with a NADH‐oxidase from Streptococcus mutans (NOx) for the kinetic resolution of pharmaceutically relevant racemic α‐chiral primary amines. The reaction conditions (e. g. pH, temperature, type of buffer) were optimised to yield S‐configured amines with up to >99 % ee.

Subjects

KINETIC resolution; GEOBACILLUS stearothermophilus; AMINES; STREPTOCOCCUS mutans

Publication

ChemCatChem, 2020, Vol 12, Issue 8, p2184

ISSN

1867-3880

Publication type

Academic Journal

DOI

10.1002/cctc.201902085