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- Title
Fluorescent DNA labeling by N-mustard analogues of S-adenosyl-L-methionine.
- Authors
Du, Yuhao; Hendrick, Charles E; Frye, Krysta S; Comstock, Lindsay R
- Abstract
Azide and alkyne-functionalized N-mustard analogues of S-adenosyl-L-methionine have been synthesized and were demonstrated to undergo efficient methyltransferase-dependent DNA alkylation by M.TaqI and M.HhaI. Subsequent labeling of the DNA with a fluorophore was carried out using copper-catalyzed azide-alkyne cycloaddition chemistry and was visualized by fluorescence scanning. This work demonstrates the utility of functionalized N-mustard analogues as biochemical tools to study biological methylation and offers a facile way to site-selectively label substrates of DNA methyltransferases.
- Publication
Chembiochem : a European journal of chemical biology, 2012, Vol 13, Issue 15, p2225
- ISSN
1439-7633
- Publication type
Journal Article
- DOI
10.1002/cbic.201200438