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- Title
Preparative, in vitro biocatalysis of triketide lactone chiral building blocks.
- Authors
Harper, Andrew D; Bailey, Constance B; Edwards, Angela D; Detelich, Joshua F; Keatinge-Clay, Adrian T
- Abstract
PKS biocatalysis: The terminal module of erythromycin synthase was used for the in vitro production of chiral triketide lactones. Combining cofactor regeneration, substrate truncation, and enzymatic promiscuity afforded a scalable strategy to generate these molecules from abundant racemic and achiral precursors. The described biocatalytic platform thus facilitates the application and study of enzymes within PKS modules.
- Publication
Chembiochem : a European journal of chemical biology, 2012, Vol 13, Issue 15, p2200
- ISSN
1439-7633
- Publication type
Journal Article
- DOI
10.1002/cbic.201200378