We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Improved L-lysine production with Corynebacterium glutamicum and systemic insight into citrate synthase flux and activity.
- Authors
van Ooyen, Jan; Noack, Stephan; Bott, Michael; Reth, Alexander; Eggeling, Lothar
- Abstract
We here developed a series of Corynebacterium glutamicum strains with gradual decreased specific citrate synthase (CS) activity and quantified in a multifaceted approach the consequences of residual activity on the transcriptome, metabolome, and fluxome level as well as on L-lysine formation and growth. We achieved an intended gradual L-lysine yield increase and recognized and overcame further new limitations in the L-lysine biosynthesis pathway to result in a strain with the highest yield reported so far when assayed under comparable conditions. As a non-intended outcome, a detailed flux analysis revealed an almost constant flux through CS at 10% remaining CS activity, whereas the metabolome data revealed an increase in the oxaloacetate and acetyl-CoA concentrations. Hence reduced CS activity is apparently efficiently buffered by increased concentrations of CS substrates, implying a certain robustness of the central metabolism in response of the imposed gene expressions.
- Publication
Biotechnology and bioengineering, 2012, Vol 109, Issue 8, p2070
- ISSN
1097-0290
- Publication type
Journal Article
- DOI
10.1002/bit.24486