Objective: To describe the knowledge of the nursing undergraduate students about the prevention of Pneumonia associated with Mechanical Ventilation and to compare this knowledge among these students in the years of 2013 and 2016. Method: Quantitative research carried out with 59 graduate students of an institution of higher education, who answered a structured questionnaire. Results: The main assertive responses were hand hygiene (94.9%), patient sedation (89.8%), oral hygiene (86.4%) and nasoenteric catheter position (83%). The Turma 2013 presented better knowledge regarding the Class of 2016 (p <0.05). Conclusions: It was concluded that students may be completing the course with a basic notion of care for the prevention of Pneumonia associated with Mechanical Ventilation, and some unobserved care are a necessary result of being reevaluated, requiring a curricular planning of the educational institutions that Facilitate the teaching-learning process.