In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate.

Imran Bashir, Khawaja Muhammad; Mohibbullah, Md.; Jeong Hyeon An; Ji-Yeon Choi; Yong-Ki Hong; Jae Hak Sohn; Jin-Soo Kim; Jae-Suk Choi

Pacific chub mackerel (Scomber japonicus) is an important fish throughout the world, especially in East Asian countries, including Korea, China, and Japan. Protein hydrolysates from marine sources are commonly used as nutritional supplements, functional ingredients, and flavor enhancers in the food, beverage, and pharmaceutical industries. Antioxidants isolated from fish are relatively easy to prepare, are cost effective, and have no reported side effects. Hence, the present study aimed to investigate the in vivo antioxidant activities of mackerel muscle protein hydrolysate (MMPH) prepared using Protamex. The in vivo bioactivities of MMPH were investigated in alcoholic fatty liver mice (C57BL/6). Serum alanine aminotransferase and aspartate aminotransferase levels were comparable in test and control mice, whereas serum triglyceride and lipid peroxidation levels significantly (p −1), especially at a concentration of 100 mg kg−1. A significant (p −1), as compared with the control group. Significantly (p −1), especially at a concentration of 100 mg kg−1. These results show that the abundant amino acids of S. japonicus play an important role in the cytosol of the liver cells by directly participating in the expression of xanthine oxidase and the detoxifying SOD and CAT proteins, thereby enhancing antioxidant ability and ultimately, inhibiting lipid peroxidation. This study demonstrated that muscle protein hydrolysate from S. japonicus has strong antioxidant activities.

NORTH Korea; JAPAN; EAST Asia; ASPARTATE aminotransferase; MUSCLE proteins; PROTEIN hydrolysates; XANTHINE oxidase; MACKERELS

PeerJ, 2018, p1

2167-8359

Academic Journal

10.7717/peerj.6181