茶多酚对糖尿病心肌病大鼠心功能的保护作用及其机制.

郑梦莹; 李 燕; 刘敬禹

Objective To investigate the protective effect of green tea polyphenol (GTP) on cardiac function in diabetic cardiomyopathy (DCM) rats and its mechanism. Methods After the model of type 1 diabetes was established by streptozotocin induction in rats, they were randomly divided into the following 5 groups for treatment: NG group (n=15), DM group (n=12), GTP group (n=12), GTP CQ group (n=11), and CQ group (n=12). NG and DM groups were given gastric lavage of distilled water. GTP group was intragastrically administered with GTP [400mg/(kg·d)] solution. CQ group was intragastrically administered with CQ solution [50mg/(kg·d)]. GTP CQ group was intragastrically administered with CQ and GTP solution of the same volume. Western blot was used to detect the expression of protein related to β-catenin/TCF4/GSK-3β and MTOR pathways in the left ventricular myocardium. Results Compared with those in NG group, the LC3-II/I/ ratio and BECN1 expression were significantly decreased (P<0.05) while SQSTM1 level was significantly increased (P< 0.05) in DM group. The LC3-II/I ratio and BECN1 protein expression level were higher in GTP group than in DM group (P<0.05), while SQSTM1 protein expression level was lower (P<0.05). The protein expressions of β- catenin, TCF4 and MYC were significantly improved in DM group as compared with NG group (P<0.05), and there were higher p-GSK3β/GSK3β and p-MTOR/MTOR ratios in DM group (P <0.05). GTP treatment significantly decreased the protein expressions of β-catenin, TCF4 and MYC, and also decreased p-GSK3β/GSK3β and p-MTOR/ MTOR ratios compared with DM group (P <0.05). Conclusion GTP participates in the protection of heart function in DCM rats by regulating β-catenin/TCF4/GSK-3β and MTOR pathways related to autophagy.

Journal of Xi'an Jiaotong University (Medical Sciences), 2018, Vol 39, Issue 6, p798

1671-8259

Academic Journal

10.7652/jdyxb201806006