肺炎克雷伯菌果糖磷酸转移酶系统 E II C 编码基因 frwC 缺失株的构建及表型分析.

林迪斯; 徐丽; 李飞雨; 汪静杰; 李蓓

Objective To construct the frwC gene deletion mutant by homologous recombination and the frwC complementation strain of Klebsiella pneumoniae NTUH-K2044 and analysis of its phenotypes. Methods The frwC gene deletion mutant was constructed by using suicide vector pKO3-Km, and then the gene fragment including frwC coding region, promoter area and transcription termination area were amplified and cloned into pGEM-T-easy plasmid to acquire frwC complementation strain. The phenotypes of the wild-type strain, the frwC mutant, and the complemented mutant were characterized. Results The frwC depletion mutant and complementation strain were constructed successfully. The growth rate and virulence of frwC mutant were decreased, but the viscosity was increased. Conclusion K. pneumoniae frwC was required for in vitro growth, capsular polysaccharide biosynthesis, and full virulence in a Klebsiella pneumoniae strain.

Journal of Xi'an Jiaotong University (Medical Sciences), 2018, Vol 39, Issue 6, p788

1671-8259

Academic Journal

10.7652/jdyxb201806004