Anti-HIV activity of ellagitannins from alder tree fruits.

Trokhymchuk, T. Yu.; Shalamay, A. S.; Zavelevich, M. P.; Palchykovska, L. G.; Vasylchenko, O. V.; Rybalko, S. L.; Starosyla, D. B.; Diadiun, S. T.

Aim. To assess the inhibitory activity of ellagitannin complex of the purified extract from the fruits of white alder and black alder in non-eukaryotic polymerase systems and to analyze the effects of these substances on the reverse transcriptase of murine leukemia virus and HIV reproduction in vitro. Methods. Chronically infected MT4/BIII culture of human leukemic cells was used as the HIV source. Anti-HIV activity of the ellagitannin-containing extract was estimated by the reduction of HIV infectious titer in the MT-4 cells. The inhibitory effects of the extract on retroviral reverse transcriptase, T7 RNA-polymerase and Taq polymerase were also studied in cell-free systems. Results. The ellagitannin extract inhibited activities of the reverse transcriptase of murine leukemia retrovirus, T7 RNA-polymerase, and Taq polymerase. It also reduced the HIV infectious titer in MT-4 cells with IC50 of 1.5 μg/ml and selectivity index of 87. Conclusions. The ellagitannin complex possesses a significant anti-HIV activity. Its in vitro antiviral effect is achieved with a relatively low toxicity.

ELLAGITANNINS; HIV infections

Biopolymers & Cell, 2018, Vol 34, Issue 3, p218

0233-7657

Academic Journal

10.7124/bc.00097C