Specific features of protein biosynthesis in higher eukaryotes.

El'skaya, A. V.; Negrutskii, B. S.; Shalak, V. F.; Vislovukh, A. A.; Vlasenko, D. O.; Novosylna, A. V.; Lukash, T. O.; Veremieva, M. V.

Over 40 years of studies in the field of higher eukaryotic translation are summarized in the review. Among the pioneer results obtained we should especially accentuate the following: i) discovery of the adaptation of tRNAs and aminoacyl-tRNA synthetases (ARSs) cellular pools to the synthesis of specific proteins and modulation of the elongation rate by rare isoacceptor tRNAs; ii) the chaperone-like properties of the translation components (ribosomes and elongation factor eEF1A); characterization of high molecular weight complexes of ARSs; iii) functional compartmentalization, including channeling of tRNA in eukaryotic cells; iv) molecular mechanisms of channeling mediated by different non-canonical complexes involving eEF1A, tRNA and aminoacyl-tRNA synthetases; v) characterization of the crystal structure of eEF1A2; vi) comparison of spatial structure, molecular dynamics, tyrosine phosphorylation and abilities to interact with different protein partners of the eEF1A1 and eEF1A2 isoforms; vii) discovery of the microRNA-mediated control of the expression of the proto-oncogenic eEF1A2 isoform in cancer cells; viii) examination of the cancer-related changes in translation elongation complex eEF1H and mechanisms of oncogene PTI-1 action; ix) discovery of the third tRNA binding site on mammals ribosomes and the allosteric interaction of the 80S ribosomal A and E sites.

TRANSFER RNA; LIGASES; BIOSYNTHESIS; PROTEIN synthesis; AMINOACYL-tRNA synthetases; AMINOACYL-tRNA; EUKARYOTIC cells

Biopolymers & Cell, 2013, Vol 29, Issue 3, p177

0233-7657

Academic Journal

10.7124/bc.000818