Organizational culture is positioned as a set of characteristics based on interactions and experiences, which produce a competitive advantage for organizations with knowledge management. The main objective of this research was focused on evaluating the organizational culture and its incidence in the knowledge management in Canuto Women's Textile Association. The methodology used focused on the bibliographic, field and correlational modalities, to diagnose the presence and relationship of the variables in the association. The analytical-synthetic methods helped in the description of the data obtained, while the deductive and inductive methods allowed the development and generation of conclusions. The evaluation of the variables was carried out through a survey focused on a validated questionnaire. The study population corresponded to a convenience sample of 22 members of the association. The results indicated the presence of unbiased values when evaluating the organizational culture, while a favorable tendency was shown in aspects such as autonomy and fluctuation of knowledge management, as well as a moderate positive relationship between both variables and their elements, except for planning. It is concluded that the association evidence limitations in the promotion of its institutional culture compared to knowledge management, where a higher level was evidenced, and it is also established that the organizational culture has a significant impact on the development of knowledge management in the association.