Spa gene Typing among Staphylococcus Aureus Isolates in Microbiology Laboratories of Scientific Research Center.

Mukhlif, Suaad S.; Taha, Mohammed; Hussein, Zainab T.; Taen, Maha M.

This study aimed to S. aureus typing using gene encoding protein A (spa gene typing) in some clinical and environmental samples collected from microbiology laboratories at scientific research center A total of 50 different environmental and clinical samples were collected from laboratories of ministry of science & technology (25 samples from environmental surfaces (benches, tables, sink,), 10 samples from mobile and 15 clinical samples. during the period from March / 2018 to May / 2018. Biochemical and morphological characterization tests showed that thirty five isolates were identified as Staphylococcus aureus of it 20 isolates from environmental surfaces, 5 isolates from mobiles, 10 isolates from researcher's hands. Antibiotic susceptibility tests of all isolates towards eight antibiotics were carried out and results showed that many isolates (25 %) were multi-resistant, DNA was extracted by wizard DNA extraction kit and it was amplified by specific primer of polymorphic X region of spa, which were determined by Ridom Staph Type software. spa typing was carried out on all positive isolates. A total of 14 spa types was detected from 35 S. aureus isolates. t008 was the most common spa type (8; 29.6%), followed by t346 (4; 14.8%), t189 (2; 7.4%), t688 (2; 7.4%), t002 (2; 7.4%). All other spa types were less than 1% of S. aureus isolates. The most common types of spa in our region were t008 (8; 29.6%), which indicate prevalence of community acquired S.aureus (CA-S.aureus) followed by t346 (4; 14.8%) from 27 spa types which were identified in this study. In conclusion, we have shown that the laboratory environment could be a reservoir contributing to dissemination of virulent S. aureus, and The proposed surveillance protocol successfully allowed the detection of S. aureus contaminating important high-touch surfaces in a representative microbiology Lab. Frequently contaminated surfaces must be targeted for routine cleaning and disinfection.

LABORATORIES; STAPHYLOCOCCUS aureus; RESEARCH institutes; MICROCOCCACEAE; MICROBIOLOGY; HEALTH resorts

Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4951

0976-0245

Academic Journal

10.5958/0976-5506.2019.03934.2