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- Title
Study of CD69 upon T-cells for SLE Iraqi Patients.
- Authors
Aziz Al-Hasan, Zahraa Saad; Ali Kanaem AL-Ibady, Qater Al-nada; Hussein, Midean Ali
- Abstract
Systemic lupus erythematosus (SLE) represented one of the chronic auto-immune multi systemic diseases affecting females more than males especially during childbearing age. A fluctuating nature of SLE disease activity deprive this disease from of any monitoring test, at likewise both of SLE disease activity index (SLEDAI) criteria or even the laboratory analyses do not confirm the disease activity, especially when a patient have normal serological results although during the disease activity (flare). This study done as a case control study, whereas the total sample size included 86 peripheral blood samples, which subdivided into: first the disease group comprised from 68 blood samples voluntary obtained from SLE patients. while the second group included 18 blood samples obtained from healthy individuals (controls) who matched in gender and age with the disease group. however, the disease group were be divided into active group (flare) and inactive group (remission) according to SLE disease activity index criteria by a specialized consultant. This study based on an immunological evaluation of CD69/CD3+ percentages of peripheral blood T-lymphocytes tested as SLE disease activity monitoring marker, owing to that purpose the CD69/CD3+ markers were be isolated from freshly blood T-lymphocytes. moreover in order to getting the best precise for Cluster Differentiation markers percentages CD69 therefore were analyzed by Immunophenotyping flow cytometer of multi-color immunofluorescent staining. Whereas the CD69/CD3+ marker ratio can be obtained from data analysis (CY flowsoftware). such ratio set up CD69test as Comparison with SLEDAI criteria, as well as with age and gender factors. whereas CD69% described as early activation marker for T-lymphocytes, so tested for such aim. the results were gave insurance about over expression of CD69Tcells for the active (flare) group. Moreover the most important indicative parameter for disease activity that systemic lupus erythematosus disease activity index whereas CD69/CD3+% has a positive correlation matching with SLEDAI score for SLE disease groups. Through these results about CD69 which proved as milestoneof SLE monitoring disease activity as independent parameter rather than SLEDAI criteria. Ultimately such results rewarded this study by finding cut off value for SLE disease activity described as CD69/CD3+ SLE activity≥ 21.5000%, within sensitivity71% and specificity 91.7% according to receiver operating characteristic curve. This study proven own aims through of the test named as CD69/CD3+ ratio marker that can be done as more easy, simplest, and accurate singular test instead of various decentralized a huge number of the clinical and laboratory investigations.
- Subjects
SYSTEMIC lupus erythematosus; RECEIVER operating characteristic curves; MATERNAL age
- Publication
Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4915
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.5958/0976-5506.2019.03928.7