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- Title
Immunopathological Study of Brucellaabortus in White Mice Immunize with Killed Antigen.
- Authors
Zakair, Khalied Yassen; Alwan, M. J.
- Abstract
The study was aimed to evaluate the protective efficiency of the formalin killed Brucellaabortusantigen against Brucella infection in mice. Inorder to achieve of thisgoal. Fifty white mice were divided into three groups randomly. The 1st group (20 mice) was immunized 0.3ml of formalin killed Brucellaabortus antigen, two doses, two weeks interval, the 2nd group (20 mice) was served as control positive while 3rd group (10 mice) was inoculated with 0.3ml of sterile normal saline and it served as control negative group. At 30 day post immunization, the 1st and 2nd group were inoculated I/P with 0.3ml of bacterial solution containing 1X108cfu/ml, I/P all animals were sacrificed at day 30 post infection, blood samples were collected for measurement IL 4, 1L six and TNFa, and small pecies from internal organs were fixed in I0 percentage of formaline for histopathological examination. The results showed that the serum levels of IL-4, IL-6and TNFα were (36.22±3.81), (29.74±2.79) and (161.214±2.98) respectevlly. The results of histopathological changes showed severe pathological changes including granulomatous lesions in the liver parenchyma and dilated of sinusoids with fibrin networks deposition, neutrophils infiltration in the lung and RBCs in thickness pleura, the spleen showed depletion of white pulp and congested red pulp as well as shownacute cellular degeneration in epithelial cells of kidney, atrophy of glomerula tufts, with dilated of Bomana spaces and hemorrhage in capsular region, while immunized infected animalswere showed mild to moderate cytopathological effect with mononuclear cells aggregation around blood vessels of examined organs particularly liver. we concluded that killed Brucellaabortous antigen can stimulated good cell mediated immune response against Brucellaabortous infection of mice.
- Subjects
CELL aggregation; MICE; ANTIGENS; EPITHELIAL cells; BLOOD vessels; BRUCELLA; CELL death
- Publication
Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4908
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.5958/0976-5506.2019.03927.5