Cytotoxic Effect of Vinca rosea Aqueous Extracts on (L20B) Cell Line In Vitro.

Obaid, Hind H.; Saqban, Liqaa H.; Mohammed, Lamia Y.

The present study investigated the cytotoxic effects of aqueous crude extracts of Vinca rosea leaves, flowers and seeds on cell line (L20B Cell line) in vitro, by using double dilution series (concentration between 1.95 -- 1000 µg/ ml. The results showed that the cytotoxic effect of extract is dependent on type of extract, amount of dose and exposure time. The concentration 1000 µg/ml gave the highest growth inhibition (IR) (74 and 74%) to leaves and flowers respectively compared with control 100% after 24 hours exposure time, but seeds were (49%) after 48 hours. However, low concentrations of aqueous extracts were found to induced the L20B cells growth and proliferation (PR), which recorded (122, 123) % by treatment with flowers extract in 1.95 µg/ml after 72 hours. Crude aqueous extract of Vinca rosea had hormetic effect (Hormesis), because it also induced the proliferation of cancer cells by using low concentrations of the extract.

CATHARANTHUS roseus; CELL lines; CELL growth; EXTRACTS; CELL proliferation

Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4881

0976-0245

Academic Journal

10.5958/0976-5506.2019.03922.6