We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
G- Protein Coupled Receptor Purification from Whole Blood of Iraqi Diabetic and Diabetic Nephropathy Patients.
- Authors
Hasan, Sulaiman M.; Al-Rubaei, Zeinab M.
- Abstract
The study aimed to purified G- Protein Coupled Receptor (GPCR) from the whole blood of control, G1, diabetic, G2, and diabetic nephropathy, G3, patients by different chromatography techniques for the first time in world that may be important in early detection and active treatment in these patients. The GPCR extracted from whole blood by precipitation of proteins with ammonium sulfate, then purified by ion exchange chromatography, as a first step using DEAE-Sepharose.A gel filtration chromatography was applied as a second step of purification using Sepharose 6B. The molecular weight and purity of GPCR was determined by electrophoresis. Results of ion exchange chromatography for G1 showed two proteins peaks that appeared after elution by the gradient concentration of sodium chloride. Data indicated that GPCR is located in the first protein peak at fraction numbers between (6 and 12). Results for G2 showed two proteins peaks that appeared after elution with the gradient concentration of sodium chloride. Then, fractions were collected and concentrated to be applied in gel filtration chromatography. Results showed a single active protein peak after eluted with elution buffer that was identical with the peak that presented GPCR concentrations at fractions number (14) for control group and show of single active protein peak that was identical with the peak that presented GPCR concentrations at fractions number (12) for G2 and G3. The molecular weight of GPCR that estimated byelectrophoresis that two bands of GPCR appear in G1at (~ 30 KD) and (~ 51 KD). Results, also, showed appearance of three bands in G2 related to GPCR in (~ 29 KD) and (~ 47, 49 KD). The conclusion could be drawn from this studythat GPCR appear in groups is a marker in detection and treatment of diabetic and diabetic nephropathy. This finding could be useful in the early detection and active treatment of diabetic and diabetic nephropathy patients based on type of GPCR that be identified.
- Subjects
DIABETIC nephropathies; PROTEIN receptors; ION exchange chromatography; GEL permeation chromatography; BLOOD proteins
- Publication
Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4853
- ISSN
0976-0245
- Publication type
Academic Journal
- DOI
10.5958/0976-5506.2019.03918.4