Background: Separating medium is a layer of coat which is applied to a mouldsurface to avoidthe adhering the second surface to the first surface. The mould must be coated during acrylic resin processing to inhibit liquid resin from penetrating into the gypsum and water from gypsum leaking to acrylic resin. For several years, tin foil was the most acceptable separating medium, and because it's challenging to apply a tin-foil substitute, a cold mold seal is utilized. Objective of this study: The aim of thecurrent study was to assess the effect of black seed oil and taramira oil on surface roughness and hardness of heat acrylic resins. Materials and Method: Sixty samples were made from the heat acrylic resins and divided into two categories (30 samples in each categories) depended on test; surface roughness and hardness, each categories contain three groups: group 1; (10) samples of heat acrylic resins treated with cold mol seal (control group), group 2; (10) samples of heat acrylic resins treated with black seed oil and group 3; (10) samples of heat acrylic resins treated with taramira oil. The average of the hardness of the specimens has been determined with using the Shore D hardness device also the average of the surface roughness of the specimens has been determined with using profilometer device. Findings: The results showed that there was a statistically significant difference in the hardness and nonsignificant difference in surface roughness of heat acrylic resinsamong the testing groups. Conclusion: In this study concluded that black seed oil gives more hardness in comparison with cold mold seal and taramira oil; also black seed oil gives more smoothness (less surface roughness) followed by taramiraoil. It is concluded, that black seed oil and taramira oil could beutilized as a separating medium.