Effect of Toxoplasma gondii Infection on DNA Sequence among patients with Testicular Cancer.

Mohammed, Ammer Abd.; Nasir, Rana S.

This study was conducted on 20 testicular cancer patients who visited the Al-Barraa privet Laboratory and Al- Sharika Labratory / Baghdad, with mean age of (27.77 ± 6.01) years. The ELISA technique was used to detect toxoplasmosis, and tumor biopsies were taken from 6 patients. The PCR amplifications of the "PIK3CA" gene were performed. The primers were used for exon 9 amplification. During the exon 9 PIK3CA gene amplification process, the first pair of the used primers included PIK3CA-9F-1, "5'-GTATTTGCTTTTTCTGTAAATCATCTG-3" and "PIK3CA-9R-1, 5' CATGCTGAGATCAGCCAAATTC-3". The determination of PIK3CA genome was done by nested polymerase Chain Reaction using primers of PIK3CA gene with amplicon size 161bp. The "oligonucleotide" was tested at 0.05, 0.075 and 0.10 μmol/L concentrations, while "LNA oligonucleotide" was tested at 0.075 and 0.0375 μmol/L concentrations for PIK3CA exon 9", which showed optimal detection for the PIK3CA exon 9 LNA mutation.

BAGHDAD (Iraq); NUCLEOTIDE sequence; TOXOPLASMA gondii; TESTICULAR cancer; GENE amplification; POLYMERASE chain reaction; CANCER patients

Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4776

0976-0245

Academic Journal

10.5958/0976-5506.2019.03905.6