The Role of Zinc Sulfate in Subchronic Cadmium Chloride Toxicosis in Male Mice.

Khudhair, Ahmed D.; Abass, Duraid A.

Aim: study sort of interaction between zinc sulfate and cadmium chloride. Method: sixty albino male mice divided equally into four groups as follows (Toxic dose of cadmium chloride 5mg/kg B.W (T1); Zinc sulfate 10mg/kg B.W (T2); their combined dosing (T3) and control group. (C) given D.W. At the end of the experiment, all the animal sacrified and their liver is dissected and prepared for histopathology also, another part of same liver submitted to homogenization process to determine the level of metallothionein 2 (MTII) and malondialdehyde (MDA). Result: histopathology of liver section revealed in the cadmium dosed group, a necrosis in hypatocyte, vascular degeneration, diffusion of mononuclear cell and neutrophill infiltration around dilated blood vessels, congestion of blood vessels around portal area and parenchyma, apoptosis of hepatocyte characterize by fragmented nuclei with irregular cytoplasm. While no lesion were recorded in liver section of zinc sulfate dosed group. In combined dosed group, liver section showed either no lesion or less severe lesion consisting of local aggregation of active macrophage and lymphocyte. Result of MTII in liver homogenate recorded highly significant increase in all treated group especially in combined dosed one that showed increase up to 3.3 times; while cadmium and zinc alone dosed group recorded increase 2.1 and 1.9 than control one. The result of MDA in liver homogenate showed highly significant increase level in cadmium toxic dosed group up to 17.8 times, while zinc sulfate group showed only 1.6 time increase than control and combined dosed group that showed no level differences. Conclusion: result of liver histopathology and MTII / MDA levels were indicative the protective role of zinc administration to overcome sub chronic cadmium toxicosis by increase their MTII binding protein that reduce the toxic effect or levels of cadmium induce cellular lipid peroxydation damage.

ZINC sulfate; CADMIUM chloride; ZINC supplements; POISONING; CARRIER proteins; BLOOD vessels; NECROSIS

Indian Journal of Public Health Research & Development, 2019, Vol 10, Issue 11, p4764

0976-0245

Academic Journal

10.5958/0976-5506.2019.03903.2