A greater rooting of crop usually allows increased growth in the plant shoots. In this sense, this study aimed to test the effect of humic acid and L-glutamic acid on the growth of the root system of the sugarcane crop. The experiment was conducted in a greenhouse with the cultivar RB867515. The evaluation of the root system was performed 90 days after sowing, through the digital image analyzer Winrhizo LA 1600®. The variables evaluated were: length, surface area, volume, diameter and dry mass of root system, height, number of leaves, leaf area and dry mass of the shoots. It was possible to conclude that the humic acids influenced the largest growth in relation to the length, surface area, volume and dry mass of the root system, differing statistically from L-glutamic acid and from the control. However, was not observed influence on the diameter of the roots and over the variables of the shoots.