Adipose-Derived Mesenchymal Stem Cells Secretome Induces Apoptosis in Colon Carcinoma HT-29 Cells.

Khodayar, Mohammad Javad; Tazangi, Fatemeh Rezaei; Samimi, Azin; Alidadi, Hadis

Background: Recent studies have demonstrated that adipose mesenchymal stem cells (AMSCs)-derived secretome (AMSC-Se) has anticancer impacts. Objectives: This study investigated the cytotoxic impacts of AMSC-Se on a colon carcinoma cell (HT-29) line. Methods: The colon cancer cells were exposed to 50 or 100g/mL ASMC-Se for 24 hours. MTT test had used to examine the impacts of ASMC-Seonthe survival rates of the cells. Caspase activity, mRNA,and protein expression of Bax and Bcl-2 had evaluated to determine apoptosis. Results: ASMC-Se could diminish the survival of the cells concentration-dependently. The mRNA and protein expression of Bax was concentration-dependently elevated, while Bcl-2 expression decreased in the ASMC-Se groupcomparedto the control concentrationdependently (P < 0.05). The caspase-3 and caspase-9 activities were concentration-dependently enhanced (P < 0.05), while the caspase-8 activity did not change with the AMSC-Se. Conclusions: These findings indicate that AMSC-Se effectively prevents cell growth and induces apoptosis by stimulating the intrinsic apoptotic pathway in these cells.

MESENCHYMAL stem cells; APOPTOSIS; COLON cancer; PROTEIN expression; CASPASES; CELL lines

Jentashapir Journal of Cellular & Molecular Biology, 2022, Vol 13, Issue 4, p1

2717-2724

Academic Journal

10.5812/jjcmb-133934