Gebelikte Tütün Dumanı Maruziyetinin Anne Sıçan Akciğer Dokusunda Meydana Getirdiği Değişiklikler Üzerine Alfa Lipoik Asitin Etkilerinin İncelenmesi.

Güzel, Elif Erdem; Kaya, Nalan; Kocamüftüoğlu, Gonca Ozan; Kısaçam, Mehmet Ali; Dabak, Dürrin Özlem; Ozan, İbrahim Enver

Objectives: The aim of our study was to investigate the effects of alpha lipoic acid on changes in the lung tissue of mothers exposed to tobacco smoke during pregnancy on an experimental rat model. Methods: In the study, twenty-eight Spraque-Dawley female rats were used. Rats were randomly divided into four groups: Control, tobacco smoke (TS), tobacco smoke alpha lipoic acid (TS ALA) and alpha lipoic acid (ALA). Rats in the TS and TS ALA groups were exposed to tobacco smoke twice a day for one hour starting from eight weeks before mating and during pregnancy. 20 mg / kg of ALA was administered to ALA and TS ALA group via oral gavage. At the end of the experiment, the rats were decapitated and lung tissues were removed and histological, biochemical and immunohistochemical methods were applied. Result: Histopathological findings such as inflammatory cell enlargement, congestion, edema, hemorrhage were observed in lung sections of TS group. Statistically significant improvement was observed in these histopathological findings with ALA application. VEGF immunoreactivity was significantly increased in TS group compared to control group and VEGF immunoreactivity decreased significantly in TS ALA group according to TS group. It was observed that the MDA levels in the group of tobacco smoke increased with respect to the control, whereas those in the TS ALA group decreased significantly with respect to the TS group. Conclusion: The oxidative damage caused by tobacco smoke in the pregnant rat lung was partially inhibited by the antioxidant effects of alpha lipoic acid treatment.

Dicle Medical Journal / Dicle Tip Dergisi, 2018, Vol 45, Issue 3, p297

1300-2945

Academic Journal

10.5798/dicletip.457249