Title: Multivariate Optimization of Separation Conditions for Simultaneous Determination of Acemetacin and Chlorzoxazone in a Pharmaceutical Preparation by HPLC Using Response Surface Methodology.
Authors: AYBABA, CIGDEM; PALABIYIK, ISMAIL MURAT; CAGLAYAN, MEHMET GOKHAN; ONUR, FEYYAZ
Abstract: The article describes a reversed phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of acemetacin (ACE) and chlorzoxazone (CHL). It highlights the use of response surface methodology (RSM) to optimize the acetonitrile and ammonium acetate percentage in the mobile phase and pH of ammonium acetate. It also discusses the reagents used in the study. The preparation of solutions of ACE, CHL and cyanocobalamin is also highlighted.
Subjects: HIGH performance liquid chromatography; ACEMETACIN; CHLORZOXAZONE; ACETONITRILE; AMMONIUM acetate
Publication: Journal of AOAC International, 2013, Vol 96, Issue 4, p723
ISSN: 1060-3271
Publication type: Academic Journal
DOI: 10.5740/jaoacint.11-092

Multivariate Optimization of Separation Conditions for Simultaneous Determination of Acemetacin and Chlorzoxazone in a Pharmaceutical Preparation by HPLC Using Response Surface Methodology.

AYBABA, CIGDEM; PALABIYIK, ISMAIL MURAT; CAGLAYAN, MEHMET GOKHAN; ONUR, FEYYAZ