Results
Title

Comparative assessment of extraction methods and quantitative HPLC estimation of Kaempferol in the leaves of Prosopis Juliflora Linn Growing in Iraq.

Authors

Jayed, Ghadeer Sadeq; Alsaad, Hiba Najeh; Al-Dallee, Zainab Tuama

Abstract

Kaempferol is an active native flavonoid, has long been recognized for its curative effects. It has been observed to possess numerous pharmaceutical characteristics. High performance liquid chromatography (HPLC) was used as a quantitative evaluation method to determine the best extraction solvent for obtaining Kaempferol from Prosopis juliflora leaves. In this study, ethanol, ethyl acetate and methanol were used as solvents in the extraction of Kaempferol flavonoid from Prosopis juliflora by employing traditional methods of extraction (reflex and maceration). The plant leaves extract of Prosopis juliflora showed existence of Kaempferol compound. Among the three solvents used, it was obvious that ethyl acetate extract of leaves showed the most significant amount of Kaempferol (92.54 mg.L-1). In accordance to the HPLC results, reflex was the most efficient technique for extracting kaempferol from Prosopis juliflora, and ethyl ethanoate was the best solvent. This information may contribute to develop this flavonoid as a possible agent for the prevention and treatment of some diseases.

Subjects

HIGH performance liquid chromatography; PROSOPIS juliflora; ETHYL acetate; SOLVENT extraction; FLAVONOIDS

Publication

Journal of Basrah Researches (Sciences), 2024, Vol 50, Issue 2, p247

ISSN

1817-2695

Publication type

Academic Journal

DOI

10.56714/bjrs.50.2.21