Results
Title

Protective Effect of Saikosaponin D against Streptozotocin-Induced Diabetic Nephropathy in Rats by Regulating the Oxidative Stress and Inflammatory Markers.

Authors

Yan Chen; Guanglu Zhang; Rijing Liang; Yao Shen; Xia Jiao

Abstract

Background: Diabetic Nephropathy (DN) is a common problem in diabetes and is characterized by glomerular dysfunction, hyperfiltration, and ultimately kidney failure. Objectives: The present study was intended to understand the salutary activities of Saikosaponin D in the Streptozotocin (STZ)-induced DN model. Materials and Methods: 60 mg/kg of STZ was administered to the rats to initiate DN, and they were then treated with Saikosaponin D (10 and 20 mg/kg, respectively) for 10 weeks. Insulin and blood glucose levels were measured using kits. The levels of renal function markers creatinine, urinary protein, Blood Urea Nitrogen (BUN), inflammatory cytokines, Malondialdehyde (MDA), antioxidant enzyme Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione Peroxidase (GPx) activities were analyzed by the corresponding assay kits. A histopathological analysis of kidney tissues was conducted on both control and treated rats. Results: The Saikosaponin D treatment remarkably reduced blood glucose while boosting body weight and insulin in the DN rats. Creatinine, urinary protein, and BUN levels were considerably reduced by Saikosaponin D. It also diminished pro-inflammatory mediators and MDA levels while boosting antioxidant enzymes. The results of the histopathological analysis also revealed that Saikosaponin D considerably ameliorated STZ-induced histological damage in kidney tissues. Conclusion: The current findings of this study demonstrate the therapeutic properties of Saikosaponin D in mitigating the development of DN in STZ-induced rats. Therefore, it was clear that Saikosaponin D could be a promising candidate to treat diabetic nephropathy.

Subjects

DIABETIC nephropathies; BLOOD urea nitrogen; BLOOD sugar; KIDNEY failure; STREPTOZOTOCIN; CREATININE

Publication

Indian Journal of Pharmaceutical Education & Research, 2024, Vol 58, Issue 3, p882

ISSN

0019-5464

Publication type

Academic Journal

DOI

10.5530/ijper.58.3.96