The effect of the amount of fat content in milk extender on ram sperm motility parameters during the storage of 5 °C for 72 h and comparison with the other semen extenders.

BÚDOVÁ, Alena; LISNER, Aleš; STRNAD, Ladislav; VOKROUHLÍKOVÁ, Jana; KUMBLE, Kateřina; BERAN, Jan

The objective of this study was to evaluate the effect of the amount of fat content in milk extender and comparison with other semen extender and evaluate their effect on ram sperm motility and kinematic parameters during the liquid storage of 5 °C for an evaluation period of 72 hours. The semen samples were collected from three Šumava sheep rams (4 years old) using an artificial vagina and were diluted by physiological solution, 0.5% fat milk, 1.5% fat milk and AndroMed® extenders with a dilution 1:100. Motility and kinematic parameters were evaluated using the CASA (Computer-assisted sperm analysis) at regular time intervals of 0, 12, 24, 36, 48 and 72 hours after sperm collection. The effect of extenders on motility and kinematic parameters varied significantly among each other. The temporal decrease rate in sperm quality differed between extenders. Physiological solution can be used on the farm for insemination within 24 hours of collection when the sperm quality is still very good. Milk diluent with 0.5% fat has better sperm motility parameters than milk diluent with 1.5% fat, but of all the extenders tested, the milk extenders had the worst quality. The best diluent tested was AndroMed®, which showed good values of motility parameters even after 72 hours of storage at 5 °C.

FAT content of milk; MILKFAT; SPERM motility; RANDOM access memory; ARTIFICIAL insemination; SEMEN; FROZEN semen

Journal of Central European Agriculture, 2024, Vol 25, Issue 4, p884

1332-9049

Academic Journal

10.5513/JCEA01/25.4.4071