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Title

Co-infection of Vibrio parahaemolyticus and Vibrio alginolyticus isolated from diseased cultured European seabass (Dicentrarchus Labrax).

Authors

Hassan Khalil R. H., Merna M. A.; T. T., Saad; Abdel-latif, H. M.; M., Tanekhy

Abstract

A total number of 100 cage-cultured European seabass (Dicentrarchus labrax) of average body weight (350.0 ±50.0 g) and average body length (30.0 ± 5.0 cm) were sampled. Fish were collected moribund and showing clinical signs from the private marine fish farm at Borg-El Arab, Alexandria province, Egypt. Fish were transferred immediately to Microbiology Laboratory at the Animal Health Research Institute (AHRI) to perform clinical, postmortem lesions (PM), and bacteriological examinations. Concurrently, water samples were collected during fish sampling to measure the different water quality parameters. Moreover, all mortalities in forty offshore cages, each cage containing 5000 European seabass, were recorded during different months. This study was directed towards providing a comprehensive overview on studying the environment-pathogen relationship in cultured seabass farms and the factors influencing the occurrence of disease. Moreover, describing the clinico-pathological picture of the bacterial isolates affecting cultured seabass during episodes of mass mortalities in the private marine farm at Borg-El Arab, Alexandria province, Egypt and make a full bacteriological investigation of the isolated Vibrio sp. Was done by morphological, colonial, biochemical characters, and VITEK 2 compact system. Additionally, evaluating the Pathogenicity challenge test by injecting the isolated Vibrio strains in the same host and determination of clinical disease in European seabass and specimens for histopathological techniques were freshly taken from the liver and spleen of the experimentally infected sea bass.

Subjects

ALEXANDRIA (Egypt); EGYPT; EUROPEAN seabass; VIBRIO alginolyticus; VIBRIO anguillarum; MIXED infections; VIBRIO parahaemolyticus; MARICULTURE; SEA basses

Publication

Alexandria Journal of Veterinary Sciences, 2022, Vol 75, Issue 2, p46

ISSN

1110-2047

Publication type

Academic Journal

DOI

10.5455/ajvs.105141

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