Activity of lactic acid-producing Streptomyces strain CSK1against Staphylococcus aureus.

Al Nuaimi, Shahad; Saadoun, Ismail; Al Joubori, Ban; Kanan, Sofian

This study aimed to evaluate an active Streptomyces strain CSK1 previously isolated from UAE soils to produce inhibitory bioactive compounds under different nutritional and growth conditions against Gram positive Staphylococcus aureus. Using a One Strain Many Compounds (OSMAC) strategy, specifically media optimization on Streptomyces strain CSK1 to enhance antibacterial activity against S. aureus, results indicated an average inhibition zone diameter ranging between 13 and 20 mm after 7 days of growth in ISP4 broth. Moreover, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the water soluble of evaporated ethyl acetate crude extract revealed values of 0.79 and 6.29 mg mL-1, respectively. GC-MS analysis of the crude ethyl acetate extract confirmed three compounds namely lactic acid, butyl lactate and lactide. The production of these compounds by Streptomyces strain CSK1 may stress their valuable importance in food and pharmaceutical industries.

STAPHYLOCOCCUS aureus; STREPTOMYCES; ETHYL acetate; LACTIC acid; BIOACTIVE compounds; ANTIBACTERIAL agents

Jordan Journal of Biological Sciences, 2023, Vol 16, Issue 2, p353

1995-6673

Academic Journal

10.54319/jjbs/160220