Peptides from Casein Extend the Survival Rate and Protect Drosophila melanogaster from Oxidative Stress Via Interacting with the Keap1-Nrf2 Pathway.

Sadiq, Idris Zubairu; Katsayal, Babangida Sanusi; Abdulazeez, Rashidatu; Adedoyin, Odunola Safiyyah; Omengwu, Yakubu Fatimat; Usman, Abdullahi Garba

It is generally established that oxidative stress has a role in the etiology of diseases linked to the lifestyle, such as cancer, aging, diabetes mellitus, and neurological diseases, among others. This research aims to evaluate the effect of casein on the survival rate and oxidative stress markers in Drosophila melanogaster. 360 flies were divided into four groups, each containing 90 flies (30 flies in triplicate). The groups include:1) the control group; 2) the H2O2 treated group; 3) the H2O2 induced + 1% Casein group; 4) the 1% Casein only group. Oxidative stress was induced in the flies and 1% Casein was used to protect the flies through enrichment of their diet and the effect of stress and Casein treatment was monitored on the mortality rate of the flies and selected oxidative stress markers. The results revealed that HR2ROR2 Rsignificantly decreases the survival rate, GSH, total thiol and total protein while it increases the level of MDA, SOD and CAT. However, treatment with 1% Casein significantly reverses the effect of H2O2 on the oxidative stress biomarkers and increases flies' survival rate. In conclusion, Casein may increase the survival rate of the fruit flies under stress and modulate stress biomarkers via possible binding of the peptides AS29, AS14, K009, and K010 to the Keap1/Nrf2 signaling pathway.

DROSOPHILA melanogaster; OXIDATIVE stress; SURVIVAL rate; FRUIT flies; PEPTIDES; CASEINS

Jordan Journal of Biological Sciences, 2023, Vol 16, Issue 2, p297

1995-6673

Academic Journal

10.54319/jjbs/160214