ANTIPROLIFERATIVE AND CYTOTOXIC POTENTIAL OF CIMICIFUGA RACEMOSA RHIZOME EXTRACT AGAINST CERVICAL CANCER CELLS.

Fatima, Shireen; Verma, Mahima; Ansari, Irfan Ahmad

Consistent human papillomavirus infection is the primary cause of uterine cervical cancer (HPV). There is a need for a more suitable and efficient treatment method due to the negative side effects of conventional chemotherapeutics used to treat advanced metatstatic cervical cancer. Recent research has shown that plant Cimicifuga racemosa (family Ranunculaceae) has powerful anti-inflammatory, antioxidant effects and anticancer effects. However, its potential against cervical cancer has not yet been clarified. Therefore, this work was aimed to examine the antiproliferative and cytotxic effects of Cimicifuga racemosa rhizome extract on human cervical cancer cells. Our results showed a dose-dependent growth inhibition in cervical cancer HeLa cells when treated with various doses of ethyl acetate rhizome extract of C. racemosa. Morpholigical analysis showed marked alterations in cancer cells including shrinking, blebbing and rounding. In addition, marked nuclear alterations in HeLa cells were observed by Hoechst 33342 staining. The H2DCFDA staining showed increased production of reactive oxygen species (ROS). Significant reduction in mitochondrial membrane potential was also observed in HeLa cells by Rhodamine123 staining. Thus the results of the study signifies the effectiveness of C. racemosa rhizome extract in cervical cancer cells, suggesting its importance as a source of potential anticancer agents for further in vitro and in vivo evaluation.

BUGBANE; CERVICAL cancer; CANCER cells; HUMAN papillomavirus; HELA cells

Biochemical & Cellular Archives, 2024, Vol 24, Issue 1, p1167

0972-5075

Academic Journal

10.51470/bca.2024.24.1.1167