Background: Anemia is one of the most common nutritional deficiency disorders which is a serious global public health problem, especially in developing countries, which is estimated to reach 30 %. Anemia is especially prevalent among adolescent girls. The prevalence of anemia according to the World Health Organization (WHO), 2017 ranges from 40-88 %. The results of Basic Health Research (Riskesdas) data in 2018 indicate that the prevalence of anemia increased from 37.1 % in Riskesdas 2013 to 48.9 % in 2018. The purpose of the study was to assess the determinants of anemia in adolescent girls aged 14-17 years at State High School (SMAN) 9 Makassar, Indonesia. Methods: The study was conducted at a State Senior High School in Makassar. With crosssectional design. A total of 211 adolescent girls were selected by proportional random sampling. Hemoglobin levels were measured using the Easy Touch GCU tool. Measurements of weight with a digital scale and height with a Microtoise height meter. Intake of fat, carbohydrate, protein, iron, folic acid, cobalamin, and ascorbic acid was obtained by 2×24-hour recall method and then calculated with nutrisurvey. Bivariate analysis used a Chi-Square test and multivariate with multiple logistic regression test. Results: Respondents who were anemic were 115 people (54.5 %). The results of the bivariate analysis showed that significant variables with anemia were protein (P=0.056; OR=1.783), iron (P=0.003; OR=2.386), cobalamin (P=0.027; OR=2.675), and ascorbic acid (P=0.010; OR=2.103). The results of multivariate analysis showed that the most influential variables on the incidence of anemia were Iron and cobalamin. Conclusion: Iron and cobalamin intake are the most influential variables in the incidence of anemia in adolescent girls at Senior High School Makassar.